EDF-1.1 is an autoclavable glass bioreactor with vessel volume 0.9 L (working volume 0.2 – 0.6 L, vessel inner diameter 85 mm).

Laboratory bioreactor EDF-1.1 is devised for cultivation of bacterial, yeast and mycelia cultures.

However bioreactor EDF-1.1 can be adapted for mammalian, stem and algae cultivation.  Depending on the used microbial culture it is possible to adapt the configuration of the bioreactor in a corresponding way by replacing stirrer and gas supply units for air, O2, N2, CO2 or different gas.

Bioreactor EDF-1.1 has a compact and ergonomic design. Temperature control of the bioreactor vessel is made by innovative temperature control system, equipped with a Peltier element. Consequently, the temperature control is provided without connecting the vessel to the bioreactor cooling water source.


Technical specification
Model EDF-1.1
Total volume (L) 0,9
Working volume (L) 0,2 – 0,6
Inner diameter (mm) 85
Inner height (mm) 168
Overall dimensions of the system (mm) 320 (W) х 400 (H) х 300 (D)
The minimum size of the autoclave chamber for vessel autoclaving (mm) 160 (Height) х 150 (Diameter)
Gas supply
Gas supply (standard) For bacterial, yeast and mycelia cultures.

Air and O2 supply using rotameters and electromagnetic valves; air flow rate range 0 – 2 slpm; O2 flow rate range 0 – 2 slpm; mixing of both gases provided. Different gas flow rate ranges can be configured on the request

For mammalian cell cultures.

Air and CO2 supply using rotameters and electromagnetic valves; air flow rate range 0 – 2 slpm; CO2 flow rate range 0 – 2 slpm; mixing of both gases provided. Different gas flow rate ranges can be configured on the request

Gas supply (option) For bacterial, yeast and mycelia cultures.

Air and O2 supply using mass flow meters; air flow rate range 0 – 2 slpm; O2 flow rate range 0 – 2 slpm; mixing of both gases provided. Different gas flow rate ranges can be configured on the request

For mammalian cell cultures.

Air, O2, N2 and CO2 supply and mixing provided using gas mix block; possibility to mix air with CO2, O2 or N2; each single gas flow rate can be controlled using rotameter (0 – 1 L/min); overall gas mix mass flow rate (0 – 0,5 L/min) are controlled with installed mass flow controller (MFC). Additional air or air / CO2 gas mix supply to bioreactor headspace and flow rate control (0 – 2 L/min) provided. Different gas flow rate ranges can be configured on the request

Agitation
Motor Top placed motor with novel magnetic drive
Impellers Two standard Rushton turbine type agitators for microbial cultures
Two “propeller” blade-stirrers for cell cultures to ensure gentle stirring regime (option)
Agitation speed (rpm) 40 – 1000
Ports
Ports For mixer / agitator shaft; for pH, pO2, T, foam and liquid level sensors; tree needle port for acid, base and anti-foam agent addition; chemo-stat tubes for level control and addition of bioreactor feed solutions; aseptic pierce-able membrane port; in-let gas port (0.2 μmn filter added); exhaust gas port (0.2 μm filter added); 2 spare ports closed with plugs. Option – aseptic sampler
Bioprocess control unit
Frame of the control unit Made from stainless steel A304, dimension 300 x 450 x 600 mm
Controller, control panel PLC- Siemens Simatic ET200SP (S7-1500 series). Digital Operator is a tablet with Windows 8 operating system
Communication 1) Ethernet connection to SCADA PC
2) Ethernet connection to LAN or WAN for remote maintenance
3)WiFi connection (VNC server) for smart phones and tablets
Sensors (standard) Temperature, pH, pO2, foam / liquid level
Sensors (option) Measuring the weight and volume of feeding components, Redox potential, pCO2, O2/CO2 gas analysis in the exhaust gas line
Temperature control Heating and cooling is controlled by an innovative system consisting of a Peltier element. Temperature control range 15 °C to 45 °C . Measurement accuracy ± 0,1 °C; accuracy of temperature within the prescribed limits ± 0,2 °C
pO2 control Polarographic sterilizable pO2 sensor (Hamilton or equivalent, length 160 mm). Automatic pO2 cascade control. pO2 measurement range: 0 – 200 % (± 1%) of saturation. Option – pO2 optical sensor
pH control Sterilizable sensor (Hamilton or equivalent, length 160 mm). Automatic control using peristaltic pumps (acid and / or base addition).  pH control range: 2 – 14 (± 0.02). Option – control via CO2 or ammonia supply
Foam and culture level control Measuring principle based on electrical conductivity measurement
Peristaltic pumps 3 freely configurable built-in pumps with digital control (ON / OFF)
Peristaltic pumps for introducing the substrate additives based on a given program (option) An optional pump may be used with a variable feeding speed, controlled by the analogue signal from the control unit
Communication and data exchange Remote access system for wireless control of the cultivation process (via Wi-Fi) with the use of smart phones or tablets
Power supply Bioprocess controller are powered using 220 V/ 50 Hz power supply
SCADA software for the collection, processing, visualization and documentation of process data (option) Computer software SCADA (basic version) for the collection, processing, visualization, archiving and documenting the process of fermentation
Computer software SCADA (validated extended version) is intended not only for the visualization and documentation of the process, but also to control the process from a remote computer, as well as with remote access via the Internet
Model based control (option) Model based fed-batch control, using algorithms programmed in MATLAB, which is connected with SCADA. User has an access to the configuration of the algorithms and addition of new control algorithms