EDF-1.1 is an autoclavable glass bioreactor with vessel volume 0.9 L (working volume 0.2 – 0.6 L, vessel inner diameter 85 mm).
Laboratory bioreactor EDF-1.1 is devised for cultivation of bacterial, yeast and mycelia cultures.
However bioreactor EDF-1.1 can be adapted for mammalian, stem and algae cultivation. Depending on the used microbial culture it is possible to adapt the configuration of the bioreactor in a corresponding way by replacing stirrer and gas supply units for air, O2, N2, CO2 or different gas.
Bioreactor EDF-1.1 has a compact and ergonomic design. Temperature control of the bioreactor vessel is made by innovative temperature control system, equipped with a Peltier element. Consequently, the temperature control is provided without connecting the vessel to the bioreactor cooling water source.
|Total volume (L)||0,9|
|Working volume (L)||0,2 – 0,6|
|Inner diameter (mm)||85|
|Inner height (mm)||168|
|Overall dimensions of the system (mm)||320 (W) х 400 (H) х 300 (D)|
|The minimum size of the autoclave chamber for vessel autoclaving (mm)||160 (Height) х 150 (Diameter)|
|Gas supply (standard)||For bacterial, yeast and mycelia cultures.
Air and O2 supply using rotameters and electromagnetic valves; air flow rate range 0 – 2 slpm; O2 flow rate range 0 – 2 slpm; mixing of both gases provided. Different gas flow rate ranges can be configured on the request
|For mammalian cell cultures.
Air and CO2 supply using rotameters and electromagnetic valves; air flow rate range 0 – 2 slpm; CO2 flow rate range 0 – 2 slpm; mixing of both gases provided. Different gas flow rate ranges can be configured on the request
|Gas supply (option)||For bacterial, yeast and mycelia cultures.
Air and O2 supply using mass flow meters; air flow rate range 0 – 2 slpm; O2 flow rate range 0 – 2 slpm; mixing of both gases provided. Different gas flow rate ranges can be configured on the request
|For mammalian cell cultures.
Air, O2, N2 and CO2 supply and mixing provided using gas mix block; possibility to mix air with CO2, O2 or N2; each single gas flow rate can be controlled using rotameter (0 – 1 L/min); overall gas mix mass flow rate (0 – 0,5 L/min) are controlled with installed mass flow controller (MFC). Additional air or air / CO2 gas mix supply to bioreactor headspace and flow rate control (0 – 2 L/min) provided. Different gas flow rate ranges can be configured on the request
|Motor||Top placed motor with novel magnetic drive|
|Impellers||Two standard Rushton turbine type agitators for microbial cultures|
|Two “propeller” blade-stirrers for cell cultures to ensure gentle stirring regime (option)|
|Agitation speed (rpm)||40 – 1000|
|Ports||For mixer / agitator shaft; for pH, pO2, T, foam and liquid level sensors; tree needle port for acid, base and anti-foam agent addition; chemo-stat tubes for level control and addition of bioreactor feed solutions; aseptic pierce-able membrane port; in-let gas port (0.2 μmn filter added); exhaust gas port (0.2 μm filter added); 2 spare ports closed with plugs. Option – aseptic sampler|
|Bioprocess control unit|
|Frame of the control unit||Made from stainless steel A304, dimension 300 x 450 x 600 mm|
|Controller, control panel||PLC- Siemens Simatic ET200SP (S7-1500 series). Digital Operator is a tablet with Windows 8 operating system|
|Communication||1) Ethernet connection to SCADA PC|
|2) Ethernet connection to LAN or WAN for remote maintenance|
|3)WiFi connection (VNC server) for smart phones and tablets|
|Sensors (standard)||Temperature, pH, pO2, foam / liquid level|
|Sensors (option)||Measuring the weight and volume of feeding components, Redox potential, pCO2, O2/CO2 gas analysis in the exhaust gas line|
|Temperature control||Heating and cooling is controlled by an innovative system consisting of a Peltier element. Temperature control range 15 °C to 45 °C . Measurement accuracy ± 0,1 °C; accuracy of temperature within the prescribed limits ± 0,2 °C|
|pO2 control||Polarographic sterilizable pO2 sensor (Hamilton or equivalent, length 160 mm). Automatic pO2 cascade control. pO2 measurement range: 0 – 200 % (± 1%) of saturation. Option – pO2 optical sensor|
|pH control||Sterilizable sensor (Hamilton or equivalent, length 160 mm). Automatic control using peristaltic pumps (acid and / or base addition). pH control range: 2 – 14 (± 0.02). Option – control via CO2 or ammonia supply|
|Foam and culture level control||Measuring principle based on electrical conductivity measurement|
|Peristaltic pumps||3 freely configurable built-in pumps with digital control (ON / OFF)|
|Peristaltic pumps for introducing the substrate additives based on a given program (option)||An optional pump may be used with a variable feeding speed, controlled by the analogue signal from the control unit|
|Communication and data exchange||Remote access system for wireless control of the cultivation process (via Wi-Fi) with the use of smart phones or tablets|
|Power supply||Bioprocess controller are powered using 220 V/ 50 Hz power supply|
|SCADA software for the collection, processing, visualization and documentation of process data (option)||Computer software SCADA (basic version) for the collection, processing, visualization, archiving and documenting the process of fermentation|
|Computer software SCADA (validated extended version) is intended not only for the visualization and documentation of the process, but also to control the process from a remote computer, as well as with remote access via the Internet|
|Model based control (option)||Model based fed-batch control, using algorithms programmed in MATLAB, which is connected with SCADA. User has an access to the configuration of the algorithms and addition of new control algorithms|