EDF-15.1 is autoclavable glass bioreactor with a working volume of 11 liters (total volume of 16 liters, the internal diameter of 200 mm).

Bioreactor is foreseen for culturing bacteria, yeast, and mycelia microorganism cultures.

However bioreactor EDF-5.4 can be adapted for culturing mammalian, stem and plant cells.  Depending on the applied microbial culture it is possible to adapt the configuration of the bioreactor in a corresponding way by replacing stirrer and a bubbling system complementing aeration gas mixing unit (air, O2, N2, CO2).

EDF-15.1 has a compact and ergonomic design. Glass cylinder alias vessel is mounted between the upper lid and the metal bottom. The bottom of the vessel has a jacket for temperature control of the vessel. It also has an autoclavable sampler for aseptic sampling. The material of the vessel, which is in contact with the product, is Pyrex borosilicate glass and stainless steel 316L. Bioreactor vessel mounted on a stainless steel legs. The lid has a convenient carrying handle. The design of the bioreactor is easy to maintain and apply basic operations and preparatory process, in particular, washing and autoclaving.

Technical specification
Model EDF-15.1
Total volume (L) 16
Working volume (L) 5 – 11
Inside diameter (mm) 200
Inside height (mm) 500
Overall dimensions of the bioreactor (mm) 1000 (W) х 970 (H) х 600 (D)
The minimum size of the autoclave chamber for vessel autoclaving (mm) 760 (Height) х 320 (Diameter)
Aeration, mixing
Aeration (standard) Supply and mixing two gases (air, O2 or air, CO2) to ensure oxygen or CO2 enrichment in headspace, including 2 pressure reducer with pressure gauges; 2 rotameters (for air 0 – 20 l/min for O2 0 – 10 l/min); microbiological filter; ring sparger (optionally microsparger); inlet gas filter; outlet gas filter with easy bending condenser; membrane valve and filter
Aeration (optionally) Separate gas supply or gas mixture to the surface of the culture
MFC for air, O2 or CO2
Gas mixing (air, N2, O2, CO2)
Mixing Top placed motor with novel magnetic drive
Impellers Two or three standard Rushton turbine type agitators for microbial cultures
Stirrers with inclined “propeller” blades for cell cultures to ensure gentle stirring regime (optionally)
Mixer rotation speed (rpm) 40 – 700
Ports
For mixer/agitator drive; for pH, pO2, T, foam and level sensors; port with three needles for introducing additives to control the pH and foam level; hemostat tube to collect the product; aseptic pierceable membrane port, a port for the incoming gas with a filter of 0.2 micron, the condenser exhaust gas with a filter of 0.2 um, 2 spare ports closed with plugs
Bottom lid Metal bottom with jackets for temperature control
Autoclavable aseptic sampler
Control and management of the culture process
Frame of the control unit Made from stainless steel A304, dimension 300x450x600 mm
Controller, control panel PLC- Siemens Simatic ET200SP (S7-1500 series);

Touch panel  Weintek eMT3070, 5.7” TFT, 800×480, 16.7M colors

Communication 1) Ethernet connection to SCADA PC
2) Ethernet connection to IAN or WAN for remote maintenance
3)WiFi connection (VNC server) for smart phones and tablets
Sensors (standard) Temperature, pH, pO2, foam, liquid level
Sensors (optionally) Optical density, measuring the weight and volume of feeding components, Redox potential, pCO2, O2/CO2 gas analysis in the exhaust gas
Temperature control Built-in thermostat (heater – 500 W, chamber, recirculation pump, cooling water valve). Temperature control range from 5 °C till 55 °C above the chiller temperature. Measurement accuracy ± 0,1 °C; accuracy of temperature within the prescribed limits ± 0,2 °C
pO2 control Polarographic sterilizable pO2 sensor (Hamilton or equivalent, length 425 mm). Optionally pO2 optical sensor. Automatic pO2 cascade control. Limits of pO2 measurements: 0 – 150 % ( ± 1%) of saturation
pH control Sterilizable sensor (Hamilton or equivalent, length 425mm). Automatic control by acid and base peristaltic pumps. Optionally via CO2 or ammonia supply. pH control range 2 – 14 (± 0.02)
Foam, liquid level control Sensors based on measuring the electrical conductivity. Level control via peristaltic pump
Peristaltic pumps for introducing technological additives 4 freely configurable built-in pump with digital control (ON / OFF), and an analog supply pump with a constant flow rate of the solution (option)
Peristaltic pumps for introducing the substrate additives based on a given program An optional pump may be used with a variable feeding speed, controlled by the analogue signal from the control unit
Communication and data exchange Remote access system for wireless control of the cultivation process (via Wi-Fi) with the use of smartphones or tablets
Power supply Part of the electric power supply, 220 V, 50 Hz and has a built-in uninterruptible power supply (UPS)
SCADA software for the collection, processing, visualization and documentation of process data (optionally) Computer software SCADA (basic version) for the collection, processing, visualization, archiving and documenting the process of fermentation
Computer software SCADA (validated extended version) is intended not only for the visualization and documentation of the process, but also to control the process from a remote computer, as well as with remote access via the Internet
Model based control (optionally) Model based fed-batch control, using supplement program, based on MATLAB and connected with SCADA trough OPC server