GALVENAA

EDF-5.4 is an autoclavable glass / stainless steel bioreactor with vessel volume of 6.2 L (working volume 2 – 4.5 L, vessel inner diameter 150 mm). Possible option – vessel volume of 8.8 L (working volume 3 – 6.3 L, vessel inner diameter 150 mm).

Laboratory bioreactor EDF-5.4 is devised for cultivation of bacterial, yeast and mycelia cultures.

However bioreactor EDF-5.4 can be adapted for mammalian, stem and algae cultivation.  Depending on the used microbial culture it is possible to adapt the configuration of the bioreactor in a corresponding way by replacing stirrer and gas supply units for air, O2, N2, CO2 or different gas.

A version of bioreactor EDF-5.4 – PBR-5.4 – is adapted for algae and cyanobacteria cultivation. PBR-5.4 additionally consists from LED cover, illumination (LED) control, air and СO2 gas supply and mixing unit.

Bioreactor EDF-5.4 has a compact and ergonomic design. Bioreactor vessel (Pyrex borosilicate glass cylinder) lower and top parts are enclosed with 316L stainless steel double wall bottom and lid, respectively, where the double wall bottom (jacket) is for thermo-stating water circulation. Bioreactor vessel is mounted on a stainless steel legs. The lid has a convenient carrying handle. The construction of the bioreactor is easy to exploit and maintain.

Technical specification
Model EDF-5.4
Total volume (L) 6.2
Working volume (L) 2 – 4.5
Inner diameter (mm) 150
Inner height (mm) 350
Overall dimensions of the system (mm) 930 (W) x 800 (H) x 600 (D)
The minimum size of the autoclave chamber for vessel autoclaving (mm) 590 (Height) x 400 (Diameter)
Gas supply
Gas supply (standard) For bacterial, yeast and mycelia cultures.

Air and O2 supply using rotameters and electromagnetic valves; air flow rate range 0 – 8.6 slpm; O2 flow rate range 0 – 8.6 slpm; mixing of both gases provided. Different gas flow rate ranges can be configured on the request.

For mammalian cell cultures, algae and cyanobacteria.

Air and CO2 supply using rotameters and electromagnetic valves; air flow rate range 0 – 8.6 slpm; CO2 flow rate range 0 – 8.6 slpm; mixing of both gases provided. Different gas flow rate ranges can be configured on the request

Gas supply (option) For bacterial, yeast and mycelia cultures.

Air and O2 supply using mass flow meters; air flow rate range 0 – 5 slpm; O2 flow rate range 0 – 2 slpm; mixing of both gases provided. Different gas flow rate ranges can be configured on the request

For mammalian cell cultures, algae and cyanobacteria.

Air, O2, N2 and CO2 supply and mixing provided using gas mix block; possibility to mix air with CO2, O2 or N2; each single gas flow rate can be controlled using rotameter (0 – 1 L/min); overall gas mix mass flow rate (0 – 0.5 L/min) are controlled with installed mass flow controller (MFC). Additional air or air / CO2 gas mix supply to bioreactor headspace and flow rate control (0 – 5 L/min) provided. Different gas flow rate ranges can be configured on the request

Agitation
Motor Top placed motor with novel magnetic drive
Impellers Two or three (6-blade) standard Rushton turbine type agitators for microbial cultures
Two or three “propeller” blade-stirrers for cell cultures to ensure gentle stirring regime (option)
Agitation speed (rpm) 40 – 1000
Ports
Ports For mixer/agitator shaft; for pH, pO2, T, foam and liquid level sensors; tree needle port for acid base and anti-foam agent addition; chemo-stat tubes for level control and addition of bioreactor feed solutions; aseptic pierce-able membrane port; in-let gas port (0.2 μm filter added); exhaust gas port (0.2 μm filter added); 2 spare ports closed with plugs. Option – aseptic sampler
Bottom lid Double wall bottom ensuring bioreactor thermostating
Autoclavable aseptic sampler
Bioprocess control unit
Frame of the control unit Made from stainless steel A304, dimension 300 x 450 x 600 mm
Controller, control panel PLC- Siemens Simatic ET200SP (S7-1500 series);

Touch panel  Weintek eMT3070, 5.7” TFT, 800×480, 16.7M colors

Communication 1) Ethernet connection to SCADA PC
2) Ethernet connection to LAN or WAN for remote maintenance
3) WiFi connection (VNC server) for smart phones and tablets
Sensors (standard) Temperature, pH, pO2, foam / liquid level
Sensors (option) Optical density, measuring the weight and volume of feeding components, Redox potential, pCO2, O2/CO2 gas analysis in the exhaust gas line
Temperature control Built-in thermostat (heater – 500 W, chamber, recirculation pump, cooling water valve). Temperature control range from cooling water temperature till 55 °C above the chiller temperature. Measurement accuracy ± 0,1 °C; accuracy of temperature regulation within the prescribed rage is  ± 0,2 °C
pO2 control Polarographic sterilizable pO2 sensor (Hamilton or equivalent, length 325 mm). Automatic pO2 cascade control. pO2 measurement range: 0 – 200 % ( ± 1%) of saturation. Option – pO2 optical sensor
pH control Sterilizable sensor (Hamilton or equivalent, length 325 mm). Automatic control using peristaltic pumps (acid and/or base addition). pH control range 2 – 14 (± 0.02). Option – control via CO2 or ammonia supply
Foam and culture level control Measuring principle based on electrical conductivity measurement
Peristaltic pumps 4 freely configurable built-in pumps with digital control (ON / OFF)
Peristaltic pumps for introducing the substrate additives based on a given program (option) An optional pump may be used with a variable feeding speed, controlled by the analogue signal from the bioprocess controller
Communication and data exchange Remote access system for wireless control of the cultivation process (via Wi-Fi) with the use of smart phones or tablets
Power supply Bioprocess controller is powered using 220 V/ 50 Hz power supply. For short term sensor data storage bioprocess controller has a built-in uninterruptible power supply (UPS)
SCADA software for the collection, processing, visualization and documentation of process data (option) Computer software SCADA (basic version) for the collection, processing, visualization, archiving and documenting the process of fermentation
Computer software SCADA (validated extended version) is intended not only for the visualization and documentation of the process, but also to control the process from a remote computer, as well as with remote access via the Internet
Model based control (option) Model based fed-batch control, using algorithms programmed in MATLAB, which is connected with SCADA. User has an access to the configuration of the algorithms and addition of new control algorithms
Illumination (LED) control (option) Illumination intensity control from 0 to 100 % PWM (0 to 1500 µmol/m2/s). Control of the illumination intensity as a time dependent profile program. Illumination with day/night cycles