Controller and bioreactor

EDF-5.4 is an autoclavable glass bioreactor with working volume till 4,5l (total volume 6,2 l, inside diameter 150 mm).

Laboratory bioreactor EDF-5.4 has two versions:

  • EDF5.4_1 for the cultivation of bacteria, yeasts and mycelial cultures;
  • EDF-5.4_2 for the cultivation of mammalian and plant cells.

 

EDF-5.4 has a compact, ergonomic and robust design. Glass cylinder vessel is mounted between the upper lid and the metallic jacketed bottom. The design of the bioreactor is easy to maintain and apply basic operations and preparatory process, in particular,
washing and autoclaving.

Novel magnetic coupling drive

Novel magnetic coupling drive

Mixer rushton turbine, pitched blades option depending from application

5L bioreactor pitched blade turbine
5L bioreactor rushton turbine

Sparger (ring sparger, microsparger) option depends on application

5l bioreactor sparger barbotier

No welded lid components – ideal for cleaning and sterility

Bioreactor top lid

Collapsible condenser allows decrease the height during autoclaving

Bioreactor condenser collapsible

Fed-batch according adjusted profiles

Bioreactor SCADA interface

Jacketed metallic bottom and bottom placed sterilizable sampler creates easy and robust operational conditions

5L bioreactor sampler

Siemens Simatic S7-1500 based PLC

Bioreactor siemens simatic controller PLC

Recipe management, archiving and other possibilities in SCADA according to the requirements of 21 CFR Part 11

5L bioreactor SCADA interface

Extension and option possibilities

Gas mixing using mass flow controllers Hamilton ARC pH and DO sensors. These sensors send 4-20 mA signals directly to PLC and ensure at the same time information management via Bluetooth in smartphones or PC installed programs. This program generates a report about all calibration procedures, sterilization numbers, predicted service life.

Connection of additional sensors: culture turbidity (optical density), culture permittivity (viable cell density), conductivity, methanol/ethanol, off-gas analysis (O2, CO2, CH4), etc.

On-line/off-line data analysis and advanced process control possibilities using PC implemented program (Matlab, Python) algorithms communicating to process control system (PLC and SCADA).

Fully equipped bioreactor vessel

Fully equipped bioreactor vessel
Technical specification
Model EDF-5.4_1 EDF-5.4_1
Usage Microbiology Cell cultures
Vessel
Total volume (L) 6,2 6,2
Working volume (L) 2 – 4,5 2 – 4,5
Inside diameter/Inside height (mm) 150/350 150/350
Aeration, mixing
Controls Rotameter, TMFC (optional) Rotameter, TMFC (optional)
Aeration Air, + O2 (optional) Air / CO2, + O2, N2 (optional) Air / CO2 in excess space
Range, l / min 0.5 – 10 0.1 – 2 (other options available)
Bubbler Ring bubbler Microsparger
Filters D51 mm 0.2 μm PTFE D51 mm 0.2 μm PTFE
Off-gas condenser Water cooling Water cooling
Drive unit type Top placed magnetic drive Top placed magnetic drive
Mixer rotation speed (rpm) 40 - 1000 40 - 500
Impellers Two standard Rushton turbines Two stirrers with inclined "propeller" blades
Control
Controller Siemens Simatic S7-1500 Siemens Simatic S7-1500
Control Panel Touch Screen Weintek eM3070, 5,7” TFT Touch Screen Weintek eM3070, 5,7” TFT
Temperature Control limits: from 5°C above cooling water up to 70 °C Measurement accuracy +/- 0.1 °C and control accuracy +/- 0.2 °C Control limits: from 5°C above cooling water up to 70 °C Measurement accuracy +/- 0.1 °C and control accuracy +/- 0.2 °C
pH Hamilton or Mettler Toledo sensor. Acid/Alkali 2-12 +/- 0.01 pH units Hamilton or Mettler Toledo sensor. Acid/Alkali and Acid/CO2 2-12 +/- 0.01pH units
pO2 Hamilton or Mettler Toledo sensor. Stir control + О2 (optional). 0-150% +/- 1% Hamilton or Mettler Toledo sensor. Stir control + gas mixtures (optional).
Foam Conductivity sensor Optional
Feeding 0.05-50 ml/min according to the set profile 0.05-50 ml/min according to the set profile
Peristaltic pumps 4 built-in variable speed pumps. Additional pumps (optional) 4 built-in variable speed pumps. Additional pumps (optional)
Dimensions
Overall dimensions of the bioreactor (mm) 930(W) х 800(H) х 600(D) 930(W) х 800(H) х 600(D)