EDF-5.4 is an autoclavable glass bioreactor with working volume till 4,5l (total volume 6,2 l, inside diameter 150 mm).
Laboratory bioreactor EDF-5.4 has two versions:
- EDF5.4_1 for the cultivation of bacteria, yeasts and mycelial cultures;
- EDF-5.4_2 for the cultivation of mammalian and plant cells.
EDF-5.4 has a compact, ergonomic and robust design. Glass cylinder vessel is mounted between the upper lid and the metallic jacketed bottom. The design of the bioreactor is easy to maintain and apply basic operations and preparatory process, in particular,
washing and autoclaving.
Novel magnetic coupling drive
Mixer rushton turbine, pitched blades option depending from application
Sparger (ring sparger, microsparger) option depends on application
No welded lid components – ideal for cleaning and sterility
Collapsible condenser allows decrease the height during autoclaving
Fed-batch according adjusted profiles
Jacketed metallic bottom and bottom placed sterilizable sampler creates easy and robust operational conditions
Siemens Simatic S7-1500 based PLC
Recipe management, archiving and other possibilities in SCADA according to the requirements of 21 CFR Part 11
Extension and option possibilities
Gas mixing using mass flow controllers Hamilton ARC pH and DO sensors. These sensors send 4-20 mA signals directly to PLC and ensure at the same time information management via Bluetooth in smartphones or PC installed programs. This program generates a report about all calibration procedures, sterilization numbers, predicted service life.
Connection of additional sensors: culture turbidity (optical density), culture permittivity (viable cell density), conductivity, methanol/ethanol, off-gas analysis (O2, CO2, CH4), etc.
On-line/off-line data analysis and advanced process control possibilities using PC implemented program (Matlab, Python) algorithms communicating to process control system (PLC and SCADA).
Fully equipped bioreactor vessel
|Total volume (L)||6,2||6,2|
|Working volume (L)||2 – 4,5||2 – 4,5|
|Inside diameter/Inside height (mm)||150/350||150/350|
|Controls||Rotameter, TMFC (optional)||Rotameter, TMFC (optional)|
|Aeration||Air, + O2 (optional)||Air / CO2, + O2, N2 (optional) Air / CO2 in excess space|
|Range, l / min||0.5 – 10||0.1 – 2 (other options available)|
|Filters||D51 mm 0.2 μm PTFE||D51 mm 0.2 μm PTFE|
|Off-gas condenser||Water cooling||Water cooling|
|Drive unit type||Top placed magnetic drive||Top placed magnetic drive|
|Mixer rotation speed (rpm)||40 - 1000||40 - 500|
|Impellers||Two standard Rushton turbines||Two stirrers with inclined "propeller" blades|
|Controller||Siemens Simatic S7-1500||Siemens Simatic S7-1500|
|Control Panel||Touch Screen Weintek eM3070, 5,7” TFT||Touch Screen Weintek eM3070, 5,7” TFT|
|Temperature||Control limits: from 5°C above cooling water up to 70 °C Measurement accuracy +/- 0.1 °C and control accuracy +/- 0.2 °C||Control limits: from 5°C above cooling water up to 70 °C Measurement accuracy +/- 0.1 °C and control accuracy +/- 0.2 °C|
|pH||Hamilton or Mettler Toledo sensor. Acid/Alkali 2-12 +/- 0.01 pH units||Hamilton or Mettler Toledo sensor. Acid/Alkali and Acid/CO2 2-12 +/- 0.01pH units|
|pO2||Hamilton or Mettler Toledo sensor. Stir control + О2 (optional). 0-150% +/- 1%||Hamilton or Mettler Toledo sensor. Stir control + gas mixtures (optional).|
|Feeding||0.05-50 ml/min according to the set profile||0.05-50 ml/min according to the set profile|
|Peristaltic pumps||4 built-in variable speed pumps. Additional pumps (optional)||4 built-in variable speed pumps. Additional pumps (optional)|
|Overall dimensions of the bioreactor (mm)||930(W) х 800(H) х 600(D)||930(W) х 800(H) х 600(D)|